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CCK-8 Cell Counting Kit for Proliferation & Cytotoxicity - Trusted China Suppliers & Factory Solutions

Introducing the Cell Counting Kit-8 (CCK8 or WST-8), a top-tier solution for cell proliferation and cytotoxicity detection. This innovative kit is based on the remarkable WST-8 compound, known scientifically as 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonobenzene)-2H-tetrazole monosodium salt, Manufactured in China, our CCK8 kits are celebrated for their rapid and sensitive detection capabilities. The functionality of WST-8 lies in its ability to be reduced by mitochondrial dehydrogenase in the presence of electron-coupled reagents. This reaction generates a highly water-soluble orange formazan, allowing for easy assessment of cell viability. The intensity of the color produced is directly proportional to cell proliferation, while inversely correlating with cytotoxic effects, As a leading supplier of laboratory products, our factory ensures high-quality standards and reliable performance. The optical density (OD) values are measured at a wavelength of 450 nm using an enzymoscope, providing an indirect yet accurate indicator of living cell counts. Experience unparalleled precision in your research with our Cell Counting Kit-8, designed for both efficiency and reliability

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    Water-Soluble Formazan High Sensitivity Wide Linear Range Superior Stability
    WST-8 is an upgrade replacement of MTT, and has obvious advantages over MTT or other MTT similar products such as XTT, MTS, etc. Firstly, Mazan, which is reduced by some dehydrogenases in the mitochondria, is not water-soluble and requires a specific solution to dissolve it. However, formazan produced by WST-8, XTT and MTS is water-soluble and can eliminate the subsequent dissolution steps. Secondly, Formazan produced by WST-8 is more soluble than Formazan produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has a wider linear range and higher sensitivity than MTT and XTT.

    CCK method is widely used in drug screening, cell proliferation assay, cytotoxicity assay, tumor drug susceptibility assay and biological factor activity detection.

    Forlab Reagent is a reagent brand under Bioland, with products including fetal bovine serum, various culture media, cryoprotectants, reagent kits, and more.

    Product Specifications
    Order NO. Description
    FL10030 100T  1 mL
    FL10030M 500T  5 mL
    FL10030L 3000T  30 mL
    FL10030XL 10000T  100 mL
    🌡️ Storage Conditions: Store at 4°C away from light. For long-term storage, keep at -20°C. Validity as indicated on the package.
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    Frequently Asked Questions
    Q What is the main advantage of WST-8 over MTT in cell viability assays?
    WST-8 produces a water-soluble formazan product, eliminating the need for the additional dissolution step required with MTT. It also offers greater sensitivity, a wider linear detection range, and superior reagent stability compared to MTT.
    Q How does WST-8 compare to XTT and MTS reagents?
    While XTT and MTS also produce water-soluble formazan, WST-8 generates a more soluble formazan product and is more chemically stable than both XTT and MTS. This improved stability leads to more reproducible and reliable experimental results.
    Q What are the main applications of the FORLAB CCK-8 Kit?
    The FORLAB CCK-8 Kit is widely used for drug screening, cell proliferation assays, cytotoxicity assays, tumor drug susceptibility testing, and biological factor activity detection.
    Q What are the recommended storage conditions for the CCK-8 Kit?
    The CCK-8 Kit should be stored at 4°C away from light for regular use. For long-term storage, it is recommended to keep the reagent at -20°C. Please refer to the product packaging for the specific validity period.
    Q What pack sizes are available for the FORLAB CCK-8 Kit?
    The FORLAB CCK-8 Kit is available in four sizes to suit different throughput needs: 100T (1 mL, Order No. FL10030), 500T (5 mL, Order No. FL10030M), 3000T (30 mL, Order No. FL10030L), and 10000T (100 mL, Order No. FL10030XL).
    Q Who manufactures the FORLAB CCK-8 Kit and what other products does the brand offer?
    The FORLAB CCK-8 Kit is produced under the Forlab Reagent brand, which is part of Bioland. In addition to the CCK-8 Kit, Forlab Reagent offers a broad range of life science products including fetal bovine serum, various cell culture media, cryoprotectants, and other reagent kits.