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China CCK-8 Cell Counting Kit Suppliers | Factory Direct Cell Proliferation and Cytotoxicity Detection

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The Cell Counting Kit-8, commonly known as CCK8 or WST-8, is a highly effective tool for the rapid and sensitive detection of cell proliferation and cytotoxicity. This kit utilizes WST-8, a chemical compound scientifically referred to as 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonobenzene)-2H-tetrazole monosodium salt, making it an essential component in cell biology research.

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How the WST-8 method works: The CCK8 kit operates by leveraging the capabilities of electron-coupled reagents that facilitate the reduction of WST-8 by mitochondrial dehydrogenase. This reaction leads to the formation of a highly water-soluble orange formazan dye. The intensity of the color produced is directly proportional to the cell proliferation rate and inversely proportional to cell cytotoxicity. By measuring the optical density (OD) at a wavelength of 450nm using an enzymoscope, researchers can indirectly quantify the number of viable cells present in their samples.

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As a leading supplier and factory in China, we take pride in offering high-quality CCK8 kits that meet the stringent standards of scientific research. Choose our reliable products to ensure accurate and reproducible results in your cell proliferation and cytotoxicity studies.

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    More Details

    WST-8 is an upgrade replacement of MTT, and has obvious advantages over MTT or other MTT similar products such as XTT, MTS, etc. Firstly, Mazan, which is reduced by some dehydrogenases in the mitochondria, is not water-soluble and requires a specific solution to dissolve it. However, formazan produced by WST-8, XTT and MTS is water-soluble and can eliminate the subsequent dissolution steps. Secondly, Formazan produced by WST-8 is more soluble than Formazan produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has a wider linear range and higher sensitivity than MTT and XTT.

    CCK method is widely used in drug screening, cell proliferation assay, cytotoxicity assay, tumor drug susceptibility assay and biological factor activity detection.

    Forlab Reagent is a reagent brand under Bioland, with products including fetal bovine serum, various culture media, cryoprotectants, reagent kits, and more.

    Product Specifications

    Order NO. Description Product Features
    FL10030 100T 1 mL Storage conditions: 4 °C away from light storage,
    long-term storage at -20 °C, the validity of the Package.
    FL10030M 500T 5 mL
    FL10030L 3000T 30 mL
    FL10030XL 10000T 100 mL

    Product Show

    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit (1)
    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit (2)
    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit (3)

    Frequently Asked Questions

    What are the advantages of WST-8 over MTT?
    WST-8 provides water-soluble formazan, eliminating the need for subsequent dissolution steps required by MTT. Additionally, WST-8 is more stable, more soluble, has a wider linear range, and offers higher sensitivity than MTT and XTT.
    What are the primary applications of the CCK method?
    The CCK method is widely utilized in drug screening, cell proliferation assays, cytotoxicity assays, tumor drug susceptibility assays, and biological factor activity detection.
    What products does the Forlab Reagent brand offer?
    Forlab Reagent, a brand under Bioland, offers high-quality products including fetal bovine serum, various culture media, cryoprotectants, and specialized reagent kits.
    How should the CCK-8 kit be stored?
    It should be stored at 4 °C away from light for regular use. For long-term preservation, it should be kept at -20 °C to maintain its validity.
    What packaging sizes are available for the FORLAB CCK-8 kit?
    The kit is available in four specifications to suit different experimental needs: 100T (1 mL), 500T (5 mL), 3000T (30 mL), and 10000T (100 mL).