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FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit

Cell Counting Kit-8, referred to as CCK8 (or WST-8) kits, is based on WST-8 (chemical name: The color reaction of 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfonobenzene) -2h-tetrazole monosodium salt is widely used in rapid and sensitive detection kit for cell proliferation and cytotoxicity

How WST-8 works: In the presence of electron-coupled reagents, it can be reduced by dehydrogenase in mitochondria to produce highly water-soluble orange formazan. The color depth is proportional to the proliferation of cells and inversely proportional to the cytotoxicity. OD values were measured at the wavelength of 450nm by enzymoscope, which indirectly reflected the number of living cells

    More Details

    WST-8 is an upgrade replacement of MTT, and has obvious advantages over MTT or other MTT similar products such as XTT, MTS, etc. Firstly, Mazan, which is reduced by some dehydrogenases in the mitochondria, is not water-soluble and requires a specific solution to dissolve it. However, formazan produced by WST-8, XTT and MTS is water-soluble and can eliminate the subsequent dissolution steps. Secondly, Formazan produced by WST-8 is more soluble than Formazan produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has a wider linear range and higher sensitivity than MTT and XTT

    CCK method is widely used in drug screening, cell proliferation assay, cytotoxicity assay, tumor drug susceptibility assay and biological factor activity detection

    Forlab Reagent is a reagent brand under Bioland, with products including fetal bovine serum, various culture media, cryoprotectants, reagent kits, and more.

    Product Specifications

    Order NO.

    Description

    Product Features

    FL10030

    100T 1 mL

    Storage conditions: 4 ° C away from light storage,

    long-term storage at -20 ° C, the validity

    of the Package

    FL10030M

    500T 5 mL

    FL10030L

    3000T 30 mL

    FL10030XL

    10000T 100 mL

    Product Show

    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit  (1)
    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit  (2)
    FORLAB Cell Counting Kit-8(CCK-8)Cell proliferation Kit  (3)
    010203

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