FORLAB RPMI 1640 Medium with L-Glutamine - China Suppliers & Factory for In Vitro Cell Culture
Product Specifications
| Order NO. | Description | Product Features | Contains Ingredients |
|---|---|---|---|
FL10011 |
RPMI1640 medium containing L-glutamine 500mL |
This product is a filtration and sterilization product |
Contain (+) |
Physical appearance: red clarified liquid |
L-glutamine |
||
Endotoxin: ≤1EU/mL |
Phenol red |
||
Osmotic pressure: 260–305 mOsm/kg·H₂O |
Excluding (−) | ||
pH value: 7.0–7.4 |
HEPES |
||
Transport condition: Blue ice |
|||
Storage conditions: 2–8 ℃, store away from light, valid for 12 months |
Frequently Asked Questions
Q
What is RPMI 1640 medium (FL10011) used for?
RPMI 1640 medium (Order No. FL10011) is a widely used cell culture medium designed to support the growth of a broad range of mammalian cells, including lymphocytes, hybridomas, and various suspension and adherent cell lines. It provides the essential nutrients, salts, vitamins, and amino acids required for cell proliferation and maintenance.
Q
What does "contains L-glutamine" mean for this product?
This formulation of RPMI 1640 includes L-glutamine as a pre-added ingredient. L-glutamine is a critical amino acid that serves as a major energy source and biosynthetic precursor for rapidly dividing cells. Having it pre-incorporated ensures consistent nutrient availability without requiring manual supplementation before use.
Q
Why does the product exclude HEPES?
HEPES is a zwitterionic buffer used to maintain pH stability in cell culture systems, especially outside of a CO₂ incubator. The FL10011 formulation does not include HEPES, making it suitable for standard incubator use with CO₂ buffering. If your application requires HEPES for extended time outside the incubator, a HEPES-supplemented variant may be more appropriate.
Q
What are the recommended storage and transport conditions?
FL10011 should be stored at 2–8 ℃ in the dark to maintain product integrity, with a shelf life of 12 months from the date of manufacture. For transport, blue ice (approximately 0–4 ℃) is required to preserve the activity of sensitive components such as L-glutamine and to prevent degradation during shipping.
Q
What quality control tests are performed on this product?
Each batch of FL10011 undergoes rigorous quality control testing, including: sterility verification through filtration sterilization, endotoxin testing (≤1 EU/mL), osmotic pressure measurement (260–305 mOsm/kg·H₂O), pH validation (7.0–7.4), and physical appearance inspection confirming a red, clarified liquid. These checks ensure consistent performance across batches.
Q
What is the role of phenol red in RPMI 1640 medium?
Phenol red is a pH indicator dye included in the RPMI 1640 formulation. It provides a visual color guide to the pH status of the medium: the medium appears red at the standard pH of 7.0–7.4, turns yellow under acidic conditions (indicating cell overgrowth or CO₂ build-up), and becomes pink to purple in alkaline conditions. This makes it easy for researchers to monitor culture health at a glance.